dextran hydrodynamic radius

The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Eng. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. (2003). European Polymer Journal, 43, 1744-1753. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. The above data are not useful because it can not be compared with those obtained in this work. Chem. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. De Belder, A.N. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. The enhanced permeability through micropores was confirmed by Franz diffusion cell. 2. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Radiofrequency with microneedling. Gold MH, Biron JA. Soc., 21, 1, 78-86. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Ahn, G.Y., Eo, HS., Kim, D. et al. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Bahary, W.S. European Polymer Journal, 36, 2143-2146. 1994, 95-0355. Clipboard, Search History, and several other advanced features are temporarily unavailable. 2003;10:33847. Nat Biotechnol. where tr is the column retention time in minutes (2 of 0.9792). J Pharm Sci. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. (2004). These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Progress in Polymer Science, 30, 38-70. Radiofrequency ablation of malignant liver tumors. National Library of Medicine Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). For Research Use Only. This site needs JavaScript to work properly. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. 2003;89(2):31120. 2012;101(8):28619. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Skin barrier disruption by acetone: observations in a hairless mouse skin model. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Hindered diffusion of dextran and ficoll in microporous membranes. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. International Journal of Pharmaceutics, 277, 99-104. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Microporation applications for enhancing drug delivery. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Journal of Controlled Release, 119, 5-24. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. Masuelli, Martin Alberto. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Journal of American Chemical Society, 76 (20), 5041-5052. Beer, M.U. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. ; Fran, H.P. Each microporated sample was incubated in the skin culture medium. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Comparison of single-point and dilution procedures. For data requests, please contact the authors. 2009;8(2):1739. (2007). Advances in Cosmetic Surgery. SWC designed and coordinated the research. Journal of Applied Polymer Science, 72, 871-876. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Epub 2021 Feb 9. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. CAS 113, 81-86. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Fixable: Fixable via Lysine. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Gillespie, T.; Hulme, M. A. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. High Manufacturing Standards of Molecular Probes Dextrans. Masuelli, Martin Alberto (2013). Journal of Physical Chemistry. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. MeSH terms The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. 2011;5(2):95132. statement and 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. 1905/05) for their financial support. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Federal government websites often end in .gov or .mil. Carbohydrate Research, 160, 205-214. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). J Control Release. Would you like email updates of new search results? In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. Food Sci Biotechnol. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Optical properties of dextran in solution and in films. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. GYA primarily conducted research. (1999). Soc., 16, 3A, 426-433. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Careers. WebR H Hydrodynamic radius [m] The basic DLS setup. There is a deviation from linearity after 580kDa [69, 70]. Pharm Res. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). and transmitted securely. Solution properties of branched dextran. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Determination of intrinsic viscosities of macromolecules and nanoparticles. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. He, L.; Niemeyer, B. 2009;6(4):34354. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Drug Discov Today Technol. Physicochemical studies of oligodextran. Progr. Sci. 2012;9(2):14754. ; Aberle, T; Burchard, W. (2000). This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Macromolecules. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Webwhittier union high school district superintendent. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC 2012;29(7):201729. Dextrans in Aqueous Solution. (1954). WebFITC Products. (1999). The genus Weissella: taxonomy, ecology and biotechnological potential. Springer Nature. Epub 2012 Oct 18. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. An undergraduate physical chemistry experiment. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. Guner, Ali (1999). Formul. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Materiaux 1-5. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Thermo Fisher Scientific. Exp Cell Res. Journal of Applied Polymer Science, 13, 2031-2032. Pharmacol Pharm. International Journal of Biological Macromolecules, 42, 133-137. The human skins were from the back or thigh of a 24-year-old woman. On single point determination of intrinsic viscosity. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. ; Bos, G.W. Curley SA. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Microporation techniques for enhanced delivery of therapeutic agents. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. With FITC-dextran 20 K, cumulative Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Walkenhorst, S.; Olivier, R. (2003). The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Article Biomaterials Research ; Grishchenko, A.E. Arch Dermatol Res. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Cite this article. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). These studies were carried out at the Hisko (Gunpo, Korea). The effect of branching is best recognized from the molecular weight dependencies of []. 43-1, 115-147. TEWL measurements were performed before and 30min after microporation. This result could reflect the presence of some aggregated species within the lysozyme sample solution. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Epub 2012 May 28. Part of Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). Critical parameters for gel permeation The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues.

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